b) From the following data on an enzymatic reaction based on competitive inhibition, determine (a) KM for the enzyme, and (b) Kị for the inhibitor-enzyme complex. Substrate Product per hour, µg...

Extracted text: b) From the following data on an enzymatic reaction based on competitive inhibition, determine (a) KM for the enzyme, and (b) Kị for the inhibitor-enzyme complex. Substrate Product per hour, µg Çoncentration, mM No inhibitor 6 mM inhibitor 88 139 179 213 2.0 121 149 3.0 4.0 10.0 313 257 370 313 15.0


Extracted text: a) The competitive inhibition model is based on the following premises: 1) 2) The enzyme lacks absolute specificity The substrate and the inhibitor compete for active site E+S+ ES k2, E+P E+I<, k_3="" ei="" 3)="" assumptions="" :="" •="" based="" on="" steady="" state="" approximation="" d(es)/="" dt="0=d(EI)/" dt="" e+p-es="" in="" negligible="" ...="" •="" e«s="" constant="" reactor="" volume="" v,="" temperature="" (t),="" and="" ph="" initial="" rate="" (no="" enzyme="" degradation)="" derive="" the="" michaelis-menten="" equation="" for="" competitive="" inhibition="" as="" the="" following="" expression:="" v="Vm(S)/{K„[1.0+(I)/K¡]+" s}="" where="" k="(k_," +k,)k,="" v,="k," e,="" k;="k_/k," i="the" inhibitor="" concentration="">