I need at least a b or higher
Brew 152 Final Examination Winter 2021 Answer on this test, then transfer answers to the answer sheet and send the answer sheet to
[email protected] 1. The Biosafety Level needed for Brew operations would be BSL-1. a. True b. False 2. Bacterial cell walls are primarily composed of which of the following? O/S 3 a. phospholipids b. protein c. carbohydrate d. peptidoglycan 3. What is the total magnification achieved if you are using the 40X objective and a 10X ocular (eyepiece)? a. 40X b. 400X c. 100X d. 1000X e. none of these 4. When measuring yeast cells using a brightfield microscope and the 100X oil immersion objective. Each graduation in the eyepiece micrometer would be about _________micron (um). See UW Stevens Point p. 42 5. If 13 ocular divisions line up with two divisions of the stage micrometer, what is the diameter (um) of a cell that spans 16 ocular divisions. 6. In preparing a smear preparation, it is important not to use too much inoculum to mix with the water and then to air dry and heat fix. a. True b. False 7. Methylene Blue and Crystal Violet are Basic (+) charged dyes that react with the microbial/yeast cells because they contain many Acidic (-) charged molecules. a. True b. False 8. Name six basic nutritional requirements supplied in all culture media. __________ ___________ _________ _________ __________ __________ 9. Why is Agar (verses Gelatin) ideal for growing microbes? See UW Stevens Point p. 5 10. Cycloheximide (water soluble) is an antibiotic use to ____________ fungi, including yeasts. 11. Chloramphenicol (ethanol soluble) is an antibiotic used to inhibit some _________ and can be added to some selective media. 12. How should agar plates be incubated and Why? ____________ _______________ 13. Review (Read) Does & Don’ts of Aseptic Technique. See UW Stevens Point, p. 12-13. 14. Why is the streak plate (e.g., dilution) a necessary part of pure culture preparations? 15. Lactobacillus and Pediococcus bacteria both stain Gram positive but can be identified easily because Lactobacillus is rod (bacilli) shaped. a. True b. False 16. After staining a smear with the Gram stain dyes, Gram positive appear red and Gram negative appear blue. a. True b. False 17. The (Endo)Spore Stain is used to identify Gram positive bacteria of the Genera Bacillus and Clostridium. a. True b. False 18. Culture characteristics are useful when working with microbes. The three characters include: Configurations, Margins, and _____________ . 19. LCSM detects for ______--______________ yeasts. 20. The catalase tests is used to identify Lactobacillus and Pediococcus bacteria due to the production and release of ____________ after the addition of Hydrogen Peroxide. 21. Identify the bacteria using the dichotomous keys (blue & pink) and the following test results: Gram + coccus, Nonmotile, Catalase + Maltose neg., Urease +, and Coagulase +. _____________ _________________ 22. If a high level of petite mutants are detected in your yeast culture, you should prepare a new _________________ . UW Stevens Point p. 30 23. Problem bacteria in brewing operations include anaerobic bacteria of the Genus Pectinatus. These Gram negative rods produce off flavors due to hydrogen sulfide and these 3 mixed acids: _____________ ________________ ________________ 24. Using the Fungal Slide Culture apparatus, why do you add sterile water to the filter paper under the glass slide containing the growing fungus? 25. When identifying fungal hyphae, the presence or absence of septum is an important characteristic. Those hyphae with septum are called coenocynic: a. True b. False 26. When performing PCR experiments, gloves are worn to prevent contamination of the sample with your own skin bacteria and degrading nucleases that degrade the template DNA or polymerase enzyme. a. True b. False 27. Which of the following is considered the most supportive media for yeast growth? a. TSY Agar b. MacConkey’s Agar c. Saborauds Dextrose Agar d. Blood Agar e. Nutrient Agar 28. When testing Water as a Quality Control Point, you should test _________ ml, weekly using a Membrane Filter Apparatus. 29. When testing a Storage tank as a Qualtiy Control Point, you should test _________ times per week. 30. To test CO2 lines, you should bubble through sterile water and filter and place onto _____________ broth. 31. Universal precautions in dealing with workplace blood spills includes: a. provides for your safety b. are protective measures designed to keep foreign body fluids from your mucous membranes c. may include the use of gloves and goggles d. a and c e. all of the above 32. When using an Autoclave, which of the following set of parameters will lead to the destruction of Bacillus subtilis an endospore former? a. the cells are destroyed by temperatures that exceed 121 degrees C. b. Endospores formed by these cells require pressure (15 psi) and heat (121 C). c. Endospores formed by these cells cannot be destroyed. d. the cells require pressure (15 psi) and heat (121 C) to be destroyed. 33. “Parfocal” refers to microscopes with multiple objectives where: a. objectives are used in pairs for stereoscopic effects b. each objective has the same working distance above the specimen c. each objective is positioned to be in focus at the same stage height. d. sequential objectives increase power by a factor of two. 34. Saccharomyces cerevisiae growing on Potato Dextrose liquid medium has an initial pH value of pH 4.9-5.0. After 24 hours of growth the pH will be: a. lower b. neutral c. higher d. the same pH as the start 35. When performing a Yeast viability count, best results are obtained using a Petroff-Hauser Counting chamber and the 100X objective. a. True b. False 36. Which of the following is considered the most supportive media for yeast growth? a. TSY Agar b. MacConkey’s Agar c. Sabourauds Dextrose Agar d. Blood Agar e. Nutrient Agar 37. Which of the following is the Gram positive control for the Gram Stain a. Staphylococcus epidermidis b. Staphylococcus aureus c. Micrococcus luteus d. Escherichia coli e. Mycobacterium smegmatis 38. A yeast cultivation medium requires 3.0 grams/liter of Glucose. How much Glucose should be added if you were to make 200 mls? a. 0.3 g b. 1.0 g c. 3.0 g d. 0.6 g e. 0.1 g 39. The most common pore size used with Membrane Filter equipment to count beer spoilage bacteria is: a. 1.0 microns b. 0.1 microns c. 4.5 microns d. 0.45 microns e. 0.22 microns 40. The Petri plate inoculated with bacteria or yeast are inverted during incubation to prevent water condensation on the larger part. a. True b. False 41. What type of chromophore is associated with a negative stain? ____________________ 42. What spoilage microbes are associated with Mashing & Wort Separation? See UW Stevens Point p. 53 ______________ _____________ & enterics __________ ___________ _________ 43. Microaerophilic organisms require Oxygen, but at a lower concentration than normally present in air. a. True b. False 44. Zymomonas mobilis and Z. anaerobia are problem bacteria in