Laboratory report title: Investigating mutation of human β-globin gene by PCR Word limit: 1200 words (only text and excluding references and Bioinformatics data) Submission deadline: 19th December...

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Laboratory report title: Investigating mutation of human β-globin gene by PCR Word limit: 1200 words (only text and excluding references and Bioinformatics data) Submission deadline: 19th December 11.30 pm, 2022 through TurnitIn. Report should contain: 1. Abstract 5 marks 2. Introduction 20 marks i. Details on Sickle cell disease. ii. Diseases linked to β-globin gene with mutations iii. Applications of PCR in investigating human diseases. iv. All these information should be supported by peer reviewed sources. v. State aim of the study. 3. Methods 10 marks i. Screenshots of Bioinformatics workflow up to in-silico PCR / Digest result ii. Brief description of PCR 4. Results 30 marks i. Bioinformatics results: Primer table with sequences and Tm, In-silico PCR and digest result, expected sizes of fragments. Brief interpretation of results. Identify another enzyme besides Bsu36I that could be used for distinguishing the WT and MT sequence of your PCR product ii. Laboratory results that are provided on the appendix for analysis: PCR and gel electrophoresis image. Correctly and completely annotated. Brief interpretation of results. 5. Discussion 25 marks i. Link your expected bioinformatics results with provided lab results ii. State and compare product sizes, presence/absence of mutation, identify WT or MT sequence iii. Comment on the control sample iv. Comment on technique used for investigation, and alternatives. v. Use your introduction and peer reviewed sources to support and strengthen your explanations. vi. Brief future work 6. List of references 10 marks i. This includes organisation of lab report 7. Provide word count Appendix: Provided lab results for investigation of investigation of human β-globin gene by PCR Into the gel, the following have been loaded in the exact order as shown in the picture: a) 5 µl 100 bp Ladder b) 10 µl Tube 1 - β-globin PCR c) 10 µl Tube 2 – Control PCR d) 10 µl Tube R – Digested β-globin PCR Laboratory results: PCR ang gel electrophoresis image • Lane 1: 100 bp ladder • Lane 2: Sample 1 PCR reaction • Lane 3: Sample 2 PCR Control • Lane 4: Restriction Digestion of PCR How does the PCR band size shown, compare to the expected band size from the in-silico PCR? Do you have a band in your control PCR lane? If so, why? Using your in-silico PCR and digest as a reference, does your sample contain the gene encoding wild-type or mutant sequence?