I performed an experiment using LPS on mouse macrophage cells and used immunofluorescence to observe the change in actin cytoskeleton. I have microscopy images of the treated and control cells, but I...

I performed an experiment using LPS on mouse macrophage cells and used immunofluorescence to observe the change in actin cytoskeleton. I have microscopy images of the treated and control cells, but I am not sure what kind of quantification and statistical testing I can perform. Any tips? I don't see how fold change would help in this case as I am not observing enhanced expression in the LPS treated cells. The actin is just elongated.
Aug 11, 2022
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