RMIT Classification: Trusted Case study Report Instructions downloadable. Practical Assessment Task 1- Case Study Report This assignment is worth 25% of the final assessment. Due Date: August 22,...


RMIT Classification: Trusted Case study Report Instructions downloadable. Practical Assessment Task 1- Case Study Report This assignment is worth 25% of the final assessment. Due Date: August 22, midnight. Length - 2000 words +/- 10% (Word count does not include references and tables). Assignment: • Students should choose one (1 only) of Case studies 1-4, from Bacterial Infections of the GIT (pages 37-41- of 2021 practical manual) and write a Case study report. • The task is to confirm the identification of the pathogen to species level using appropriate enterics workflows, media and biochemical tests, and write a report on the case. • The cultures and biochemical tests will be performed in class in weeks 3&4. You will also be required to : • Write a brief clinical lab report to the physician on your findings. Use the reporting template provided. Faeces Report ( and if you choose the S. typhi case- Blood culture) • Write a report on your chosen case study and identification. You are required to describe the method, workflow and results of your identifications, and to discuss your findings. In addition, your discussion should research and discuss the symptoms, pathogenesis and recommended treatment of the pathogen you identify. Detailed instructions are given below- please be sure to read them. These instructions can be downloaded in PDF format:Case study Report instructions >>to be updated Reference materials The following have been provided for download from the Canvas site to assist you with your assignment. You may already have hard copies of these if you have taken a microbiology class at RMT before: Identification tables for Bacteria RMIT Techniques Manual Report Format Taksh Patel RMIT Classification: Trusted The following headings should be used for your Case Study report: • Cover sheet • Introduction • Materials and Methods • Results • Laboratory Report Lab report template- Faeces.docx • Discussion • References (Vancouver format). Please note correct formatting and referencing are part of the assessment criteria and marks will be deducted if format is not correct. Cover sheet. Please include a cover sheet with your name, student number, and the Case number you are reporting on. (A complete School of Science coversheet is not necessary) Case Study Report sections, important points on what to include: Introduction The introduction should include general background information including: the significance of gastrointestinal tract infections, reasons for performing microbiological investigations on these patients, the importance of correct testing of antibiotic sensitivities, and expected outcome or benefit from such investigations. The introduction should end with a brief statement that summarises the nature of the case that was investigated. Materials and Methods The protocol used in your own investigations should be explained and justified, this should be written in the past tense. eg. “Overnight cultures on HBA were examined and suspicious colonies were picked for identification”.. The methods section should be written so that the work could be repeated by another student. For this type of report, you do not need to list materials used as these are standard and may be found in any microbiology laboratory. Any test procedures described in detail in the RMIT Techniques Manual, or other such as the Manual of Clinical microbiology, or Melbourne University Techniques Manual should be simply referenced by giving the source and page number (Vancouver style, include a page number in references to Technical manuals). You should also note any kits or commercial test kits that were used, including the manufacturer. The simplest way to present this information is in a table or point format for example: RMIT Classification: Trusted Eg. The following tests were used to identify organisms in this study. Methods were as described in the RMIT Techniques Manual (1) or the Manual of Clinical Microbiology (2) as indicated. For organism A the following tests were used A: • Catalase test(1)p24 • DNAse test(1)p56 • Hippurate test(2) Note: Many Students have used the online GERMM database at RMIT in previous classes. These methods are a great reference in the lab, but this is not an appropriate reference for these reports because it is not easily accessible, and because web links are not an appropriate reference for this type of document. You are required to refer to an easily accessible source with page numbers- for example the RMIT technical manual, or the University of Melbourne Technical manual. The test descriptions in the RMIT technical manual are almost identical to those in the GERMM. Students are advised to use a copy of the RMIT techniques manual. Note: The methods section of your report will probably be very similar so other students- this is not a problem. Results Your results should be organised and presented as clearly as possible, avoiding repetition. Tables and diagrams are usually preferable to long descriptions of positive and negative test results. Tables should have clear headings and any abbreviations should be explained below the table. You should note the gram stain result and any growth conditions, (eg. O2 or AnO2) or growth on any specialist or selective media, if relevant. You do not need to describe the colour change or appearance of the tests, simply record whether the results was positive or negative, or gas production etc. For example: Summary of test results for Case 12. Gram positive cocci. Test Result Growth in air + Growth on MCA + Lactose fermentation on MCA - Growth on HBA + Haemolysis on HBA α Lancefield group D Optochin R RMIT Classification: Trusted Make sure that your results section includes a statement of what the organism you identified is! Laboratory Report to Requesting Physician Use the template provided to complete the 1-page laboratory report (download template from the assignment page on the Canvas site). This report should only contain the information that would be reported back to the clinician. Correct reporting terminology will be discussed in the practical classes. State the relevant patient and specimen details and present your results clearly and concisely. Methods used to obtain those results are the responsibility of the laboratory and are not included in the report. Do not list antimicrobials used for identification purposes only. Discussion The discussion section should provide an interpretation of the results, including any unusual findings or difficulties in the identification. It should not contain extensive repetition of the results or of information in the introduction. A good way to discuss the results is to point out the critical tests that made it possible to identify the organism to genus and species level for example. If you had any unexpected results you should provide explanation, and discuss how it could have been done better. “Organism A was identified to species level using from four biochemical tests. Using the gram stain appearance, and the growth on MCA, it was possible to identify the organism as member of the Streptococci (Table 1, (3)). Then the presence of alpha haemolysis, susceptibility to Optochin permitted identification of Streptococus pneumoniae (Table 4 (3))." Make sure you discuss your findings in the context of the case notes you received. How does the organism you identified fit with the patient type, symptoms etc. The discussion section must also include a short discussion of the significance of the pathogen that was isolated in the case study under investigation, with appropriate reference to the scientific literature (approx. 7-10 references in Vancouver style). Your discussion should address the following points for each pathogen you isolated: • How is the infection usually acquired? • Symptoms, duration and possible complications of infection • Brief discussion of how pathogenesis of infection leads to the symptoms • Usual treatment recommendations and the implications for the patient in the case study you have chosen. References : RMIT Classification: Trusted In the course of preparing your report and researching the pathogen you have identified it is expected that you will have read some of the scientific literature on the subject. This might include a textbook,but should also include peer-reviewed journal articles, and/or a clinical laboratory manual. Web links from non- peer reviewed sources are NOT appropriate for a scientific report. Use the Vancouver format for your references. Please see the reference list for my document below for examples. Submitting your Case Study Report • Please combine all parts of your assignment into one word or PDF document and submit using the Turnitn link . FAQ. Turnitin scores: All students will be using similar methods so some parts of your report are likely to be similar to other students. As a guide we would expect Turnitin scores to be <15% similarity for this type of report. if your score is higher than this, you should review which sections are similar and make changes. if the only similarity is in the materials and methods, or references section then a little over 15% is ok. it is important that your introduction and discussion are in your own words. remember that we can see the turnitin reports when we mark and are able to judge. scores over 20% are not acceptable. you will need to revise your report and resubmit. remember that a turnitin report will take about 24 hours soon your 3rd submission to turnitin. general formatting requirements for scientific reports: • organism names are always italicised. • the first time an organism is mentioned by name, the name must be written out in full. afterwards, the shortened version is used, for example, escherichia coli then e. coli. • the same applies to other abbreviations, for example calibrated dichotomous sensitivity first then cds. you should use an abbreviation such as cds only if it comes up at least five times in the text; otherwise write out in full. • be careful with the use of capitals. the only words that are capitalised mid- sentence are names of people, places or companies (proper nouns). incorrect usage; …. “were plated onto horse blood agar (hba)” correct usage …. “the organisms were plated onto horse blood agar (hba)”. references for these guidelines. these similarity="" for="" this="" type="" of="" report.="" if="" your="" score="" is="" higher="" than="" this,="" you="" should="" review="" which="" sections="" are="" similar="" and="" make="" changes.="" if="" the="" only="" similarity="" is="" in="" the="" materials="" and="" methods,="" or="" references="" section="" then="" a="" little="" over="" 15%="" is="" ok.="" it="" is="" important="" that="" your="" introduction="" and="" discussion="" are="" in="" your="" own="" words.="" remember="" that="" we="" can="" see="" the="" turnitin="" reports="" when="" we="" mark="" and="" are="" able="" to="" judge.="" scores="" over="" 20%="" are="" not="" acceptable.="" you="" will="" need="" to="" revise="" your="" report="" and="" resubmit.="" remember="" that="" a="" turnitin="" report="" will="" take="" about="" 24="" hours="" soon="" your="" 3rd="" submission="" to="" turnitin.="" general="" formatting="" requirements="" for="" scientific="" reports:="" •="" organism="" names="" are="" always="" italicised.="" •="" the="" first="" time="" an="" organism="" is="" mentioned="" by="" name,="" the="" name="" must="" be="" written="" out="" in="" full.="" afterwards,="" the="" shortened="" version="" is="" used,="" for="" example,="" escherichia="" coli="" then="" e.="" coli.="" •="" the="" same="" applies="" to="" other="" abbreviations,="" for="" example="" calibrated="" dichotomous="" sensitivity="" first="" then="" cds.="" you="" should="" use="" an="" abbreviation="" such="" as="" cds="" only="" if="" it="" comes="" up="" at="" least="" five="" times="" in="" the="" text;="" otherwise="" write="" out="" in="" full.="" •="" be="" careful="" with="" the="" use="" of="" capitals.="" the="" only="" words="" that="" are="" capitalised="" mid-="" sentence="" are="" names="" of="" people,="" places="" or="" companies="" (proper="" nouns).="" incorrect="" usage;="" ….="" “were="" plated="" onto="" horse="" blood="" agar="" (hba)”="" correct="" usage="" ….="" “the="" organisms="" were="" plated="" onto="" horse="" blood="" agar="" (hba)”.="" references="" for="" these="" guidelines.="">
Aug 18, 2021

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