XXXXXXXXXXTable 1: Standard and Unknown Protein Samples and Their Absorbance BI101 Latest 2014.pdf 57 DETERMINATION OF PROTEIN CONCENTRATION IN MILK PRODUCTS OBJECTIVES 3. Calibrate and use a...

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Table 1: Standard and Unknown Protein Samples and Their AbsorbanceBI101 Latest 2014.pdf57DETERMINATION OFPROTEIN CONCENTRATIONIN MILK PRODUCTSOBJECTIVES3. Calibrate and use a spectrophotometer.4. Plot a “standard” curve.5. Use a standard curve to determine the protein concentration of several milk products.INTRODUCTIONqualitative measurement of the presence of proteins. quantitative proteins bind to a dye called Coomassie Blue that is used in the Bradford protein assay. This Determining protein concentration is of importance to biologist studying enzymes. Enzymes are 7Name___________________________Lab Section___________________________58Lab Exercise 7RELEVANCE The ability to precisely measure quantities of nutrients such as proteins is important in quantitatively assess foods enabling us to tailor diets to the needs of patients, the elderly, and your results to nutritional labels. PROCEDUREconcentration of protein for each solution, a graph results. This is called a standard curve. spectrophotometer.Micropipette Practice:dispensing volumes from 10-100 I. STANDARD CURVE AND SAMPLE PREPARATIONconcentration. 1. Mix the tube of Coomassie Blue dye solution by inverting 3-4 times. 2. Next, use the micropipette of 1 mL capacity to add 3 mL of Coomassie Blue dye solution to 9 spectrophotometer cuvettes labeled 0, 1, 2, 3, 4, 5, M1, M2, and M3.L of each protein standard 0, 1, 2, 3, 4, and 5 using the 10-100 L micropipette into the corresponding cuvettes by Be sure to use a fresh tip for each sample.59Protein Concentration in MilkTable 1: Standard and Unknown Protein Samples and Their AbsorbanceCuvette LabelType of ProteinSampleCoomassieBlue SampleProteinConcentration0 Standard 3 mL 60 L of tube 01 Standard 3 mL 60 L of tube 12 Satndard 3 mL 60 L of tube 23 Standard 3 mL 60 L of tube 34 Standard 3 mL 60 L of tube 45 Standard 3 mL 60 L of tube 5M1 3 mL 60 L of tube M1M2 3 mL 60 L of tube M2M3 3 mL 60 L of tube M3M1, M2 and M3 respectively by touching the tip of the pipette to the inside of the cuvette Be sure to use a fresh tip for each sample.5. vortex each tube so that the mixture produces a uniform color. inaccurate data.II. DETERMINING THE PROTEIN CONCENTRATION OF UNKNOWN SAMPLES1. Using absorbance data from Table 1, create a standard curve by plotting the absorbance 60Lab Exercise 7Absorbance values0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0Protein concentration (mg/mL)Table 2: Unknown Milk Protein SamplesSamples ValuesEstimated Protein M1M2M361Protein Concentration in MilkIII. CALCULATIONSTable 3: True Concentrations of Unknown SamplesSampleEstimate of ProteinConcentration from Multiplied by the Dilution Factor EqualsFinal Concentration M1 × 50M2 × 50M3 × 50actual composition of the samples if presented nutritional labels from several milk products. Table 4: Converted Concentrations of Unknown SamplesSample Final Concentration Multiplied by the Conversion Factor EqualsConcentrationM1 ×M2 ×M3 ×7 g per serving. This value is directly comparable to the provided nutritional labels. Using this 62Lab Exercise 7Table 5: Serving-Size Concentrations for the Unknown SamplesSample Concentration Times Number of mL per serving EqualsConcentrationM1 ×M2 ×M3 ×actual identities to the class.Table 6: Probable Identities of Unknown SamplessampleCalculated class average for the Probable milk item Were you M1M2M3IV. QUESTIONSText1: Text2: Text3: Text4: Text5: Text6: Text7: Text8: Text9: Text11: Text10: Text13: Text12: Text14: Text15: Text16: Text17: Text18: Text19: Text20: Text21: Text23: Text24: Text25: Text26: Text27: Text28: Text29: Text30: Text31: Text32: Text33: Text22: Text34: Text35: Text36: Text37: Text38: Text39: Text40: Text41: Text42: Text43: Text44: Text45: Text46: Text47: Text48: Text49: Text50: Text51: Text52: Text53: Text54: Text55: Name: Lab Section:
Answered 1 days AfterJun 19, 2022

Answer To: XXXXXXXXXXTable 1: Standard and Unknown Protein Samples and Their Absorbance BI101 Latest 2014.pdf...

Preeti answered on Jun 21 2022
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BI101 Latest 2014.pdf
57
DETERMINATION OF
PROTEIN CONCENTRATION
IN MILK PRODUCTS
OBJECTIVES
3. Cali
ate and use a spectrophotometer.
4. Plot a “standar
d” curve.
5. Use a standard curve to determine the protein concentration of several milk products.
INTRODUCTION
qualitative measurement of the presence of proteins.
quantitative
proteins bind to a dye called Coomassie Blue that is used in the Bradford protein assay. This
Determining protein concentration is of importance to biologist studying enzymes. Enzymes are
7
Name___________________________
Lab Section___________________________
58
Lab Exercise 7
RELEVANCE
The ability to precisely measure quantities of nutrients such as proteins is important in
quantitatively assess foods enabling us to tailor diets to the needs of patients, the elderly, and
your results to nutritional labels.
PROCEDURE
concentration of protein for each solution, a graph results. This is called a standard curve.
spectrophotometer.
Micropipette Practice:
dispensing volumes from 10-100
I. STANDARD CURVE AND SAMPLE PREPARATION
concentration.

1. Mix the tube of Coomassie Blue dye solution by inverting 3-4 times.

2. Next, use the micropipette of 1 mL capacity to add 3 mL of Coomassie Blue dye solution
to 9 spectrophotometer cuvettes labeled 0, 1, 2, 3, 4, 5, M1, M2, and M3.
L of each protein standard
0, 1, 2, 3, 4, and 5 using the 10-100 L micropipette into the co
esponding cuvettes by
Be sure
to use a fresh tip for each sample.
59
Protein Concentration in Milk
Table 1:...
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