This assignment requires an analysis to find if the population in the given data is inbreeding. Im just wondering if its possible to get the assignment back within 5 hours!
Microsoft Word - Assignment1.docx Assignment1,BIOL4390,PopulationGenetics,Prof.A.Zayed Jan2021 Assignment1[33.33%] DueBYFridayFebruary5th2021[latesubmissionswillnotbeaccepted] Thisassignmentwillinvolveanalysisofseveralsimulatedpopulationgeneticdatasetstopractice someoftheconceptsdiscussedinlectures.Eachfilecontainsdatafromseveral populations/datasets;YOUONLYNEEDTOANALYSETHEDATASETSASSIGNEDTOYOUIN DATA_ASSIGNMENT.TXT! WriteDataset#sonfirst/titlepageofyourassignment[1mark] Thelectureswillprovideyouwithalltheneededknow-howtoanswerthequestions.Havefun andbringupanyquestionsyouhaveabouttheassignmentduringclass,oronthediscussion forum. Allozymegeneticsexercise(14marksintotal): [Data_Source]:Allozyme_genotypes.txt;Thedatafileconsistsofdiploidgenotypesfor20fruit fliesat5allozymeloci(EST1toEST5)sampledfrommanypopulations.Pleasefindthe populationassignedtoyou(Data_Assignment.txt).Eachdistinctalleleatalocusisgivenaunique number(e.g.12=heterozygousindividualwithallele1andallele2).Thereisamaximumof3 allelesperlocus. Analysethedatatoestimatetheparametersbelow 1. Allelefrequencies(p1,p2,p3) 2. NumberofAlleles/Locus,a 3. ObservedHeterozygosity/locusHobs 4. ExpectedHeterozygosity/locusHexp[2marks] 5. Proportionatereductioninheterozygosityduetoinbreeding,Fis[2marks] 6. x2forHWEtest[2marks] 7. dfforHWEtest[2marks] 8. pvalueforHWEtest[2marks] Providemewithacompletedtablelikebelow;report2decimalplacesforallestimates,butkeep 3decimalplaceswhiledoingthecalculations POP_# EST1 EST2 EST3 EST4 EST5 p1 P2 P3 A Hobs Hexp Fis HWE,x2 HWE,df HWE,p Isyourpopulationinbreeding:Yes/No[1marks] Backupyouanswerwithevidence(2to3lines):[3marks] Assignment1,BIOL4390,PopulationGenetics,Prof.A.Zayed Jan2021 Sequenceanalysisexercise:(5marksintotal) [Data_Source]:Sequence_diversity.txt;Pleasefindthedatasetassignedtoyou. You’vesequenced1000bpofyourfavouritegenefrom5haploidindividuals.Thedataset specifiesallpolymorphicpositionsinthesequences(theirlocationdoesnotmatterhere). Estimatethefollowingparameters; 1.ProportionofSegregatingSites,S[3decimalplaces][1mark] 2.Numberofmismatchesateachsegregatingsite,presentedinthesameorderasinthedataset andseparatedbycommons[eg:3,4,4,3,…][2marks] 3.Nucleotidediversity,π[4decimalplaces][2marks] Dataset_54 Sequence length: 1000 The following sites are segregating: 14 T G G C C C C T G C C G C A G G C C G A A G A C C G A A G A C G G A A G A C C T G C G A C C C G C G T C C C G C G T C C C G G A T T G C A G Est 1 Est 2 Est 3 Est 4 Est 5 22 22 22 22 22 22 22 23 22 22 22 22 22 22 22 22 22 32 22 21 22 23 22 22 22 23 22 23 22 22 22 32 22 22 22 22 22 22 22 22 32 22 22 22 22 32 12 12 22 21 22 22 22 22 22 23 22 22 22 21 22 23 22 22 22 22 22 22 22 22 22 22 12 22 22 23 22 12 22 22 22 22 22 22 21 32 22 22 22 22 22 22 22 22 22 22 22 21 22 22