A biochemist discovers and purifies a new enzyme, generating the purification table below. (a) From the information given in the table, calculate the specific activity of the enzyme after each...

A biochemist discovers and purifies a new enzyme, generating the purification table below.

(a) From the information given in the table, calculate the specific activity of the enzyme after each purification procedure.
(b) Which of the purification procedures used for this enzyme is most effective (i.e., gives the greatest relative increase in purity)?
(c) Which of the purification procedures is least effective?
(d) Is there any indication based on the results shown in the table that the enzyme after step 6 is now pure? What else could be done to estimate the purity of the enzyme preparation?

Extracted text: Procedure Total protein (mg)Activity (units) 1. Crude extract 2. Precipitation (salt) 3. Precipitation (pH) 4. Ion-exchange chromatography 5. Affinity chromatography 6. Size-exclusion chromatography 20,000 4,000,000 5,000 3,000,000 4,000 200 1,000,000 800,000 50 750,000 45 675,000