Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an...


Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme






















































Test




Unknown Isolate B




1



Luminescence




Positive




2



Cell Shape




Straight Rods




3



Gram Staining




Negative




4



Oxygen Requirement




Facultative Anaerobe




5



Motility           (Hanging


Drop)




Motile




6



Growth at


0% NaCl




Negative




7



3% NaCl




Positive




  8



6 % NaCl




Positive


















































































































9



Accumulation of PHB




Positive




10



Flagella




1 Polar




11



Oxidase




Positive




12



Nitrate Reduction






13



Gelatinase




Negative




14



Growth at 35OC




Positive




15



Growth at 4OC




Negative




16



Methyl Red




Positive




17



Voges-Proskauer




Positive




18



Catalase




Positive




19



Starch Hydrolysis




Negative




20



Lysine Decarboxylase




Negative




21



Indole Production




Negative




22



Utilization of






23



Glucose




Positive (fermentative)




24



Lactose




Positive (fermentative)




25



Mannitol




Negative




26



Sucrose




Negative




27



Xylose




Positive (fermentative)




28



Inositol




Negative




  29



Galactose




Positive (oxidative)





Jun 02, 2022
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