EFFECT OF ENZYΜΕ CONCENTRATION OΝ ENZΥME ACTIVITY Question Guide: 1. Approximately what is the concentration of the enzyme in each tube? Show computation Tube No. Computation 1 2 3 4 5 6.

Extracted text: EFFECT OF ENZYΜΕ CONCENTRATION OΝ ENZΥME ACTIVITY Question Guide: 1. Approximately what is the concentration of the enzyme in each tube? Show computation Tube No. Computation 1 2 3 4 5 6.


Extracted text: Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.