I have a quiz that I have attached that needs to be doneThank you
Quiz 1 Biochemistry 473 winter term 2019________________________ 1) 2 points Please write out how to make this solution from the stocks given. Make 1 L 10 mM Tris pH8 5% SDS 1 mM EDTA 2) 5 points Compare your results from week 1 and 2, describing the saturation curve (if obtained) and what concentrations you were studying of Avidin and HABA. Write up the methods section of the binding assay in journal format 3)5 points Choose one graph of receptor competition assay. (end of quiz) Write out an assay that might have been used to get the results. (Show details) What are your conclusions of this experiment? Compare the structures of the compounds and explain why this data might have resulted from structural comparison. 4) 3 points Create a nice image of avidin using the protein data bank. Provide information on structure number, name, reference, and display. PPMA competition of [3H]N-methylspiperone binding to wild-type D2, wild-type D4, and single- (A), double- and triple- (B), and multiple- (C) residue mutant D2 receptors. A, dose-response curves for D2 (●), D2V91F (▴), D2F110L (▪), D2Y408V (♦), and D4 (○). B, dose-response curves for D2 (●), D2V91F/Y408V (▾), D2F110L/Y408V (▵), D2V91F/F110L (■), D2V91F/F110L/Y408V (▿), and D4 (○). C, dose-response curves for D2 (●), D2V91F/F110L/V111M/Y408V (∗), D2W90L/V91F/liter94S/F110L/V111M/Y408V (⋄), and D4 (○). The assays were performed as described in Experimental Procedures. The means ± S.E. (duplicate determinations) are shown from a representative experiment performed three times or more with similar results. The data are fit to a variable-slope, one-site competition model by nonlinear regression Cells were incubated for 10 min with 10 μM forskolin and 100 μM 3-isobutyl-1-methylxanthine in the absence or presence of increasing concentrations of dopamine or aripiprazole. cAMP was measured using the Biotrak cAMP Direct Screening Assay System (Amersham Biosciences). Accumulation of cAMP stimulated by forskolin ranged from 3 to 11 pmol/well. Data were fit to a four-parameter logistic equation using GraphPad Prism version 3.0 (GraphPad Software, San Diego, CA). The data shown are the mean ± S.E.M.,n = 8 (CHO-D2L) and 5 (HEK-293-D2L; inset) experiments. The density of D2L receptors was determined by saturation binding of [3H]spiperone to washed membranes prepared from CHO-D2L cells previously incubated in the absence or presence of EEDQ at 37°C for 60 min. Nonspecific binding was determined with 2 μM (+)-butaclamol. KDand Bmax values were determined by fitting data to a one-site binding isotherm.