1 Assessment Brief Assessment Brief_L4_489_2021/22 Read this assessment brief carefully, it tells you how you are going to be assessed, how to submit your assessment on-time and how (and when) you’ll...

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Assessment Brief
Assessment Brief_L4_489_2021/22

Read this assessment
ief carefully, it tells you how you are going to be assessed, how to
submit your assessment on-time and how (and when) you’ll receive your marks and

Module Code ASC_4_489_2122
Module Title Microbiology
Lecturer Valentina Caputo
% of Module Mark 30%
Distributed 28/01/2022
Submission Method Submit online via this Module’s Moodle site
Submission Deadline
CW1 (30%) 29/04/22 11:59pm. Before the deadline, you
need to ensure that the document you have uploaded is
the co
ect one. No changes will be accepted after the
deadline without EC or penalties

Release of Feedback Feedback will be available online from 20/05/2022
Release of Marks
Provisional marks will be available in the Gradebook on
Moodle from 20/05/2022
Lab Report. Each student will be given one activity (or technique) to do a
comprehensive lab report about.
Assessment Details:
Assessment of your work will consider

A. Content, quality and organization of your scientific writing.
You have an opportunity to apply the subject knowledge that you have acquired and
einforced during this semester.
This will be assessed according to the following standards (see the marking criteria
at the end of the document):

1. Research
Systematic Identification and use of academic and relevant resources.

2. Subject Knowledge
Understanding and application of subject knowledge. Contribution to subject
debate by updated research

3. Critical Analysis
Analysis and interpretation of sources, literature and/or results. Structuring of

XXXXXXXXXXPractical Competence.
Skills to apply theory to practice or to test theory

B. The quality of your scientific writing

Structure, quality and clarity of argument, coherence, use of evidential support and
eferencing, spelling, grammar and punctuation.
This will be assessed according to the following strands:

7. Communication and Presentation
Clear intention in communication. Audience needs are predicted and met.
Presentation format is used skillfully. Work is well structured.
8. Academic Integrity
Acknowledges and gives credit to the work of others follows the conventions
and practices of the discipline including appropriate use of referencing
standards for discipline.


Type: Lab Report
Resources: Lab handbook
Lab tutorials
1. Cappuccino JG and Welsh C. Microbiology: a
laboratory manual (2018)

Scientific literature
Word Count: Lab report. As a guide, aim for 1200 words. The
maximum word limit is 1400 words.
References, tables and appendixes will not count
towards word count totals
If the total word limit is exceeded, a 5% penalty will apply
for every 5% extra.
Presentation: Your lab report must contain:
Title and student number
Introduction (definition of the main subject). Give
the general background of where the technique is
applied. Explain the principle of the technique, the
situations when the technique is used and why it is
important. Define the objective of the practical.

Method. Step by stem description of how the
method works. Mention the sensitive steps and the
potential source of variations.
Asses the safety precautions required.

Describe and interpret your results. Report your
esults (be
ief). Interpret them and discuss what
they could mean and why. Here is where you need
show a deep understanding of what you have done.
If things did not work, mention the possible causes
and how to address them (troubleshooting).
Answer ALL the question related to your activity in
the lab handbook (mark the questions individually).

Compare and contrast. Research for other /novel
techniques/methodologies used to for the same
measurement. Why there are bette
Compare time, sensitivity, selectivity
The lab report must have figures, pictures, graphs
and tables to help explain concepts and summarize
information. Number of figures/tables and add the
Figure legend
 Work must be referenced
 Work must be submitted as a unique Word document
(.doc/docx). The name of the document MUST
include the name or number of the activity you are
 Course work should be done in Arial font size 11 (or
larger if you need to), with a minimum of 1.5 line
spacing. All the pages need to be numbered.
 Your student numbers must appear at the front of
the coursework. Your name must not be on your
Referencing: Harvard Referencing should be used, see your Li
Subject Guide for guides and tips on referencing.
Regulations: Make sure you understand the University Regulations on
expected academic practice and academic misconduct.
Note in particular:
▪ Your work must be your own. Markers will be
attentive to both the plausibility of the sources
provided as well as the consistency and approach to
writing of the work. Simply, if you do the research
and reading, and then write it up on your own, giving
the reference to sources, you will approach the work
in the appropriate way and will cause not give
markers reason to question the authenticity of the
▪ All quotations must be credited and properly
eferenced. Paraphrasing is still regarded as plagiarism
if you fail to acknowledge the source for the ideas
eing expressed.

TURNITIN: When you upload your work to the Moodle
site it will be checked by anti-plagiarism software. 15%
Turnitin score is the maximum allowed.


Learning Outcomes
This assessment will fully or partially assess the following learning outcomes for this

Knowledge and Understanding
Develop understanding of ascetic techniques and general microbiology techniques
ent and potential uses of different techniques in different settings
Intellectual Skills
Explain the fundamental principles, techniques and applications on microbiology
Transferable Skills
Develop skills in research and critical scientific writing
Demonstrate the ability to work safely and independently in laboratory settings
How to get help

We will discuss this Assessment Brief in class. However, if you have related
questions, please contact the lecturers in the MS TEAMS site for the Microbiology
module as soon as possible.

Lab handbook
Lab tutorials
Reading list
Open access scientific literature
Technical references (equipment and reagents)

Breakdown of criteria:
Quality Exceeds Expectations (4) Meets Expectations (3) Acceptable (2) Unacceptable (1)
Content (70%)
Explanation of
significance (15%)
Present, co
ect, complete. Writing is
dynamic, and engages ready attention
Present, adequately complete, co
Explanation is well written, contains
asic info.
Present, somewhat incomplete, some
ors. Contains necessary info, but
Not present or very limited, or major
ors. Significance of research is lost.
Summary of
esearch findings
Main conclusions, supporting and
evidence are fully explained and are
ect interpretations of the paper
Main conclusions, supporting evidence
are adequately explained, and mostly
ect with no or minor technical e
in interpretation.
Main conclusions and supporting
evidence are partially explained, or have
some inco
ect interpretations.
Main conclusions and supportive
evidence are minimally explained, or
there are some major e
ors in
Inclusion of
information (10%)
Relevant background is fully included
and is co
Relevant background information is
present at adequate levels, and is
Relevant background information is
insufficient, or moderately inco
Relevant background information is
missing or highly inco
Explanation of
figure/graph/ table
Strong choice of figure, is fully
explained, and is a co
ect interpretation
Reasonable choice of figure, is
adequately explained, and is a co
interpretation with no or minor technical
Reasonable choice of figure, is partially
explained, and has some inco
No figure given, or minimal/no
explanation. May have major e
ors in
Format and style (30%)
References and
use of direct
quotations (10%)
In-text Harvard style citations are used
ectly where required. No more than
two quotations are used.
In-text Harvard style citations are used
ectly where required, with no more
than two minor formatting e
ors. No
more than two quotations are used.
In-text citations are used where
equired, in an inco
ect style; and/or,
three quotations are used.
Outside material/information is not cited;
and/or more than four quotations are
Structure (5%) Between 725 and 775 words, with
ect margins and font. Written in
clear paragraphs, structured in inverted
pyramid format.
Between 725 and 775 words, with minor
ors in margins or font. Written in
clear paragraphs, structured in inverted
pyramid format.
More than 775 words or less than 725
words. E
ors in margins, font, and/or
paragraph structure. Inverted pyramid
elements are present, but not in co
More than 825 or less than 675 words.
ors in margins, font, /or and
paragraph structure. Lacks any
organizational structure. Essential
information is lost.
Language level
and clarity (10%)
Always comprehensible without having
to refer to original paper or other
sources; any specialized vocabulary is
defined and necessary; language is
neither too technical nor too simple.
Almost always comprehensible without
having to refer to original paper or other
sources; any specialized vocabulary is
defined and necessary;
Answered Same DayApr 06, 2022


Preeti answered on Apr 07 2022
16 Votes
Selective Media: MacConkey Aga
1. Introduction
Discovery of culture medium by Louis Pasteur has led to the development of microbiology (Wainwright and Lede
erg, 1992). A culture medium is a mixture of nutrients, amino acids etc. that allows the growth of microorganisms or cells for example, LB (Luria-Bertani) medium for the growth of bacteria, DMEM (Dulbecco Minimal Essential Medium) for the growth of mammalian cells etc. There are basically six different types of media – Basal, Enriched, Indicator, Transport, Storage and Selective Media. Selective media is a growth medium that allows the growth of required or certain organisms and inhibits the growth of undesired organisms for example, MacConkey Agar, Lowenstein-Jensen media etc. MacConkey Agar is a selective medium, discovered by Alfred T MacConkey, that favours the growth of gram-negative bacterium only and the appearance of the microbial growth based on their ability of metabolising lactose (Elazhary et al., 1973). The medium is mainly composed of crystal violet dye, bile salts, lactose, and neutral red as pH indicator (Lagier et al., 2015). The bile salts and crystal violets present in the medium can differentiate between the gram-positive and gram-negative bacteria as they inhibit the growth of gram-positive bacteria. The presence of pH indicator in MacConkey medium is used to differentiate further between lactose fermenters and non-ferments gram-negative bacterial species. The lactose-fermenter bacteria will produce pink colonies while non-lactose fermenter bacteria will grow as off-white colonies. The lactose fermenters bacteria can further be differentiated on MacConkey agar medium by observing their rate of growth and on the presence of capsule. Different bacteria grows at different rates and the capsulated bacteria also grows differently than non-capsulated bacteria. The bacteria that ferments the lactose weakly, will grow slowly than strong lactose fermenters. The encapsulated bacteria forms sticky and wet appearing colonies and hence they can easily be differentiated from non-capsulated bacterial species.
2. Methods
For the preparation of MacConkey agar medium, dehydrated powder of medium was taken. 49.53 grams of medium (Peptones – 20g, Lactose monohydrate – 10g, 0.15% Bile salts, 0.5% NaCl, 0.03 g Neutral red, 0.001g Crystal violet, 13.5g Agar) was weighed and dissolve in 1 litre of distilled water. The mixture was heated until it dissolved completely. It was further autoclaved at 121oC, for 20 minutes and then poured in Petri dishes. The Petri dishes were allowed to cool and then used further for the streaking of bacteria.
Three different...

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