1 Assessment Brief Assessment Brief_L4_489_2021/22 Read this assessment brief carefully, it tells you how you are going to be assessed, how to submit your assessment on-time and how (and when) you’ll...

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Selective media: MacConkey


1 Assessment Brief Assessment Brief_L4_489_2021/22 Read this assessment brief carefully, it tells you how you are going to be assessed, how to submit your assessment on-time and how (and when) you’ll receive your marks and feedback. Module Code ASC_4_489_2122 Module Title Microbiology Lecturer Valentina Caputo % of Module Mark 30% Distributed 28/01/2022 Submission Method Submit online via this Module’s Moodle site Submission Deadline CW1 (30%) 29/04/22 11:59pm. Before the deadline, you need to ensure that the document you have uploaded is the correct one. No changes will be accepted after the deadline without EC or penalties Release of Feedback Feedback will be available online from 20/05/2022 Release of Marks Provisional marks will be available in the Gradebook on Moodle from 20/05/2022 Assessment: Lab Report. Each student will be given one activity (or technique) to do a comprehensive lab report about. Assessment Details: Assessment of your work will consider A. Content, quality and organization of your scientific writing. 2 You have an opportunity to apply the subject knowledge that you have acquired and reinforced during this semester. This will be assessed according to the following standards (see the marking criteria at the end of the document): 1. Research Systematic Identification and use of academic and relevant resources. 2. Subject Knowledge Understanding and application of subject knowledge. Contribution to subject debate by updated research 3. Critical Analysis Analysis and interpretation of sources, literature and/or results. Structuring of issues/debates. 6. Practical Competence. Skills to apply theory to practice or to test theory B. The quality of your scientific writing Structure, quality and clarity of argument, coherence, use of evidential support and referencing, spelling, grammar and punctuation. This will be assessed according to the following strands: 7. Communication and Presentation Clear intention in communication. Audience needs are predicted and met. Presentation format is used skillfully. Work is well structured. 8. Academic Integrity Acknowledges and gives credit to the work of others follows the conventions and practices of the discipline including appropriate use of referencing standards for discipline. 3 Type: Lab Report Resources: Lab handbook Lab tutorials 1. Cappuccino JG and Welsh C. Microbiology: a laboratory manual (2018) Scientific literature Word Count: Lab report. As a guide, aim for 1200 words. The maximum word limit is 1400 words. References, tables and appendixes will not count towards word count totals If the total word limit is exceeded, a 5% penalty will apply for every 5% extra. Presentation: Your lab report must contain: Title and student number Introduction (definition of the main subject). Give the general background of where the technique is applied. Explain the principle of the technique, the situations when the technique is used and why it is important. Define the objective of the practical. Method. Step by stem description of how the method works. Mention the sensitive steps and the potential source of variations. Asses the safety precautions required. Describe and interpret your results. Report your results (be brief). Interpret them and discuss what they could mean and why. Here is where you need show a deep understanding of what you have done. If things did not work, mention the possible causes and how to address them (troubleshooting). Answer ALL the question related to your activity in the lab handbook (mark the questions individually). Compare and contrast. Research for other /novel techniques/methodologies used to for the same measurement. Why there are better/different? Compare time, sensitivity, selectivity The lab report must have figures, pictures, graphs and tables to help explain concepts and summarize information. Number of figures/tables and add the Figure legend 4  Work must be referenced  Work must be submitted as a unique Word document (.doc/docx). The name of the document MUST include the name or number of the activity you are reporting  Course work should be done in Arial font size 11 (or larger if you need to), with a minimum of 1.5 line spacing. All the pages need to be numbered.  Your student numbers must appear at the front of the coursework. Your name must not be on your coursework. Referencing: Harvard Referencing should be used, see your Library Subject Guide for guides and tips on referencing. Regulations: Make sure you understand the University Regulations on expected academic practice and academic misconduct. Note in particular: ▪ Your work must be your own. Markers will be attentive to both the plausibility of the sources provided as well as the consistency and approach to writing of the work. Simply, if you do the research and reading, and then write it up on your own, giving the reference to sources, you will approach the work in the appropriate way and will cause not give markers reason to question the authenticity of the work. ▪ All quotations must be credited and properly referenced. Paraphrasing is still regarded as plagiarism if you fail to acknowledge the source for the ideas being expressed. TURNITIN: When you upload your work to the Moodle site it will be checked by anti-plagiarism software. 15% Turnitin score is the maximum allowed. https://libguides.lsbu.ac.uk/subjects/home https://libguides.lsbu.ac.uk/subjects/home http://www.lsbu.ac.uk/__data/assets/pdf_file/0008/84347/academic-regulations.pdf 5 Learning Outcomes This assessment will fully or partially assess the following learning outcomes for this module. Knowledge and Understanding Develop understanding of ascetic techniques and general microbiology techniques Current and potential uses of different techniques in different settings Intellectual Skills Explain the fundamental principles, techniques and applications on microbiology Transferable Skills Develop skills in research and critical scientific writing Demonstrate the ability to work safely and independently in laboratory settings How to get help We will discuss this Assessment Brief in class. However, if you have related questions, please contact the lecturers in the MS TEAMS site for the Microbiology module as soon as possible. Resources Lab handbook Lab tutorials Reading list Open access scientific literature Technical references (equipment and reagents) 6 Breakdown of criteria: Quality Exceeds Expectations (4) Meets Expectations (3) Acceptable (2) Unacceptable (1) Content (70%) Explanation of significance (15%) Present, correct, complete. Writing is dynamic, and engages ready attention Present, adequately complete, correct. Explanation is well written, contains basic info. Present, somewhat incomplete, some errors. Contains necessary info, but awkward. Not present or very limited, or major errors. Significance of research is lost. Summary of research findings (30%) Main conclusions, supporting and evidence are fully explained and are correct interpretations of the paper Main conclusions, supporting evidence are adequately explained, and mostly correct with no or minor technical errors in interpretation. Main conclusions and supporting evidence are partially explained, or have some incorrect interpretations. Main conclusions and supportive evidence are minimally explained, or there are some major errors in interpretation. Inclusion of background information (10%) Relevant background is fully included and is correct Relevant background information is present at adequate levels, and is correct Relevant background information is insufficient, or moderately incorrect. Relevant background information is missing or highly incorrect Explanation of figure/graph/ table (15%) Strong choice of figure, is fully explained, and is a correct interpretation Reasonable choice of figure, is adequately explained, and is a correct interpretation with no or minor technical errors Reasonable choice of figure, is partially explained, and has some incorrect interpretations No figure given, or minimal/no explanation. May have major errors in interpretation. Format and style (30%) References and use of direct quotations (10%) In-text Harvard style citations are used correctly where required. No more than two quotations are used. In-text Harvard style citations are used correctly where required, with no more than two minor formatting errors. No more than two quotations are used. In-text citations are used where required, in an incorrect style; and/or, three quotations are used. Outside material/information is not cited; and/or more than four quotations are used. Structure (5%) Between 725 and 775 words, with correct margins and font. Written in clear paragraphs, structured in inverted pyramid format. Between 725 and 775 words, with minor errors in margins or font. Written in clear paragraphs, structured in inverted pyramid format. More than 775 words or less than 725 words. Errors in margins, font, and/or paragraph structure. Inverted pyramid elements are present, but not in correct order. More than 825 or less than 675 words. Errors in margins, font, /or and paragraph structure. Lacks any organizational structure. Essential information is lost. Language level and clarity (10%) Always comprehensible without having to refer to original paper or other sources; any specialized vocabulary is defined and necessary; language is neither too technical nor too simple. Almost always comprehensible without having to refer to original paper or other sources; any specialized vocabulary is defined and necessary;
Answered Same DayApr 06, 2022

Answer To: 1 Assessment Brief Assessment Brief_L4_489_2021/22 Read this assessment brief carefully, it tells...

Preeti answered on Apr 07 2022
108 Votes
Selective Media: MacConkey Agar
1. Introduction
Discovery of culture medium by Louis Pasteur has led to the development of microbiology (Wainwright and Lederberg, 1992). A culture medium is a mixture of nutrients, amino acids etc. that allows the growth of microor
ganisms or cells for example, LB (Luria-Bertani) medium for the growth of bacteria, DMEM (Dulbecco Minimal Essential Medium) for the growth of mammalian cells etc. There are basically six different types of media – Basal, Enriched, Indicator, Transport, Storage and Selective Media. Selective media is a growth medium that allows the growth of required or certain organisms and inhibits the growth of undesired organisms for example, MacConkey Agar, Lowenstein-Jensen media etc. MacConkey Agar is a selective medium, discovered by Alfred T MacConkey, that favours the growth of gram-negative bacterium only and the appearance of the microbial growth based on their ability of metabolising lactose (Elazhary et al., 1973). The medium is mainly composed of crystal violet dye, bile salts, lactose, and neutral red as pH indicator (Lagier et al., 2015). The bile salts and crystal violets present in the medium can differentiate between the gram-positive and gram-negative bacteria as they inhibit the growth of gram-positive bacteria. The presence of pH indicator in MacConkey medium is used to differentiate further between lactose fermenters and non-ferments gram-negative bacterial species. The lactose-fermenter bacteria will produce pink colonies while non-lactose fermenter bacteria will grow as off-white colonies. The lactose fermenters bacteria can further be differentiated on MacConkey agar medium by observing their rate of growth and on the presence of capsule. Different bacteria grows at different rates and the capsulated bacteria also grows differently than non-capsulated bacteria. The bacteria that ferments the lactose weakly, will grow slowly than strong lactose fermenters. The encapsulated bacteria forms sticky and wet appearing colonies and hence they can easily be differentiated from non-capsulated bacterial species.
2. Methods
For the preparation of MacConkey agar medium, dehydrated powder of medium was taken. 49.53 grams of medium (Peptones – 20g, Lactose monohydrate – 10g, 0.15% Bile salts, 0.5% NaCl, 0.03 g Neutral red, 0.001g Crystal violet, 13.5g Agar) was weighed and dissolve in 1 litre of distilled water. The mixture was heated until it dissolved completely. It was further autoclaved at 121oC, for 20 minutes and then poured in Petri dishes. The Petri dishes were allowed to cool and then used further for the streaking of bacteria.
Three different...
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