Bio Sci 325 Exam 2 Summer 2012
If you are unclear about what is called for, ask for help (e-mail) if anything is unclear. Good luck. These problems are each worth 5 points
1. Consider the following transduction experiment: donor: a+ b+ c+ recipient: a- b- c-selected marker: a+ # of colonies:
a+ b+ c+ 6 a+ b- c- 65 a+ b+ c- 0 a+ b- c+ 12
Give the gene order:
1. Pt 2 Consider the following transformation experiment: After transformation, strepr cells are selected and tested for growth in minimal media. 10 colonies were arg+ and 45 colonies were leu+
Donor: Recipient:
strepr, arg+, leu+ streps, arg-, leu-
Which gene is closer to strep?
2. Consider the following timed mating experiment:
hfr: strepr arg+ pro+ his+ leu+ F-: strepr arg- pro- his- leu-
GENES TRANSFERRED strepr arg pro his leu time 0 s - - - - in 1 s - + -min 2 s - + + -3 s - + + + 4 r - + + + 5 r + + + +
Give the correct order of these genes relative to the F plasmid:
1