Essay 1.Compare and contrast the use of plasmids and bacteriophage vectors in gene cloning. Your...

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Essay 1.Compare and contrast the use of plasmids and bacteriophage vectors in gene cloning. Your answer should include diagrams of named vectors, a description of standard protocols for their usage and an analysis of the advantages and disadvantages associated with each vector type.Essay 2.Analyse the features of the polymerase chain reaction which have made it an essential tool for scientists from biomedical research to forensic investigations. Include; descriptions of the components of a PCR reaction and the thermocycle, analysis of the specificity and sensitivity of PCR and discussion of named applications of PCR.			Document Preview:				Essay 1.  Compare and contrast the use of plasmids and bacteriophage vectors in gene cloning.  Your answer should include diagrams of named vectors, a description of standard protocols for their usage and an analysis of the advantages and disadvantages associated with each vector type.  Essay 2.  Analyse the features of the polymerase chain reaction which have made it an essential tool for scientists from biomedical research to forensic investigations.  Include; descriptions of the components of a PCR reaction and the thermocycle, analysis of the specificity and sensitivity of PCR and discussion of named applications of PCR.	Essay 1. Compare and contrast the use of plasmids and bacteriophage vectors in gene cloning.  Your answer should include diagrams of named vectors, a description of standard protocols for their usage and an analysis of the advantages and disadvantages associated with each vector type. Essay 2. Analyse the features of the polymerase chain reaction which have made it an essential tool for scientists from biomedical research to forensic investigations.  Include; descriptions of the components of a PCR reaction and the thermocycle, analysis of the specificity and sensitivity of PCR and discussion of named applications of PCR.

Robert · Accepted Answer

Running head MOLECULAR GENETICS: AN AMAZING BRANCH OF SCIENCE
Molecular Genetics: An Amazing Branch of Science 
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Molecular Genetics: An Amazing Branch of Science 
Answer 1 
Vectors: The carrier molecules 
 Vector might be defined as; vehicle that can be used for transportation of a molecule 
from one position to another. To solve the purpose, diverse types of vectors can be applied by 
researchers for instance; Plasmid, Bacteriophage, Yeast Artificial Chromosome (YAC), and 
Bacterial Artificial Chromosome (BAC) etc. Out of above, the key characteristics of Plasmid and 
Bacteriophage have been defined as follows:  
Plasmid: A Renowned Vector  
 Plasmid can be observed in its host in the form of a small, double-stranded, extra-
chromosomal, and closed-circular DNA.  It is considered as; a valuable tool by molecular 
biologists because of its tiny size, and presence of origin of replication as well as marker(s) in it. 
Origin of replication is essential for duplication of plasmid and markers are required for its 
selection. Besides, small size of plasmid is also advantageous in terms of handling. In addition to 
above, clone producing ability (in terms of number of clones and time required) of plasmid is 
quite appreciable since; it can generate several clones within a limited time-frame (Fire & Xu, 
1995).  
How Plasmid is applied?  
 For the purpose of cloning, plasmids are first isolated from their host- cell so that, these 
might be cut at specific site with the assistance of Restriction Endonuclease (R.E) enzyme. Then 
the gene isolated from donor is introduced into plasmid and ligase enzyme is used to close the 
gap. Now, this recombinant moiety can be inserted into the host via application of transformation 
tools and techniques (for example, by treating the plasmid and/or host cell with chemicals). After 
being introduced into the host cell, plasmid can produce several copies of inserted fragment  
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Molecular Genetics: An Amazing Branch of Science 
through replication that can further be isolated and purified.  
                                      Amp
R 
(Ampicillin Resistance gene)
    Tet
R               3273 BP
 
  (Tetracycline Resistance gene) 
                                                            pBR327 (3,273 bp)
            Ori 
                                      (Replication origin)            R.

Essay 1. Compare and contrast the use of plasmids and bacteriophage vectors in gene cloning. Your answer should include diagrams of named vectors, a description of standard protocols for their usage...

Answer To: Essay 1. Compare and contrast the use of plasmids and bacteriophage vectors in gene cloning. Your...

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