I need about 5 pages including with a reference.Metabolic Biochemistry GST and the Effects of...

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I need about 5 pages including with a reference.Metabolic Biochemistry GST and the Effects of Curcumin Practical:  Criterion-Referenced Marking Criteria Rubric  You will be required to present class data in a laboratory report. In the week following the practical session there will be a tutorial  covering elements of the report write up. Report submission will occur the week after the tutorial.        The report  demonstrates an  understanding of the  scientific concepts  associated with the  topic being studied.  Weight 20%.  The report is  presented as  specified in the  provided  guidelines within  the Subject  Manual. Weight  20%.  The laboratory  results are  presented in an  appropriate manner.  Weight 5%.  References are  cited in the  prescribed  method (i.e.  within the text  and in the  reference list).  Weight 5%.  The statistical  analyses have been  performed correctly.  Weight 10%.  The information  within the report has  been presented in a  coherent and concise  manner that is clear  to the reader.  Weight  10%.  Students have  critically analysed the  experimental results  and associated  literature to draw  reasoned conclusions  from the data. Weight  30%. Disciplinary knowledge  Criterion High distinction Distinction Credit Pass Fail  The report demonstrates an  understanding of the scientific  concepts associated with the topic  being studied. Weight 20%.   Outstanding report overall.   Very well written  introduction.    Concisely covers the function  of GST, previous studies with  curcumin, why curcumin  might be significant and can  describe the tissue  localisation of GST.   Includes aims that are  appropriately written in  concise scientific manner.   Coherently and logically links  the data to our  understanding of the subject  matter. Very well referenced.   Excellent report  overall.   Well written  introduction.    Covers GST and  curcumin.   Includes well written  aims.   Discussion is  comprehensive but  does not draw on the  literature much.   Some minor  referencing  inconsistencies.   Very good report  overall.   Introduction does not  sufficiently (in detail)  cover GST and  curcumin.    Aims are not included  or described poorly.   Discussion is brief and  does not cover all the  results.    There is little  literature used  throughout the  report.   Referencing is  minimal   Good report  overall.   Introduction is  brief and does  not cover all the  main points  covered in this  practical.   Written without  an  understanding  the concepts.   Some  misconceptions  about the  experimental  aims are evident.    Discussion is very  brief and shows  some lack of  understanding of  the results.   Little effort to  draw upon the  literature.   Not a good  report that is  missing vital  information.   Introduction  does not cover  GST or  curcumin.   Introduction is  too brief.   Poorly written  with a clear  lack of  understanding  of the  experimental  aims.    Parts of the  results are  missing.   Discussion is  too brief and  with little  reference to  the results or  literature.   Professional skills  Criterion High distinction Distinction Credit Pass Fail  The report is presented as  specified in the provided  guidelines within the Subject  Manual. Weight 20%.    NB: Margins are to be at least  2.5cm top, bottom, left and right.  Font is to be Times New Roman 12  point or Ariel 11 point.   The cover sheet and  declaration have been  completed.   Kept within the specified  page limit.   Table titles have been  included above the tables.   Figure legends have been  included below the figures  and are understandable in  isolation of text.   Used correct axis labels with  appropriate units.   Error bars included on the  graph are representative of  experimental standard  deviation.   Professional presentation  with great attention to detail.   Significant p-values indicated  with an asterisk in the table  and/or on the graph.   Fonts used are appropriate    Margins are within limits   The cover sheet and  declaration have been  completed.   Like HD level but  lacking in some aspects  of the presentation.   Adequate and correct  presentation of figures  and tables.   Figure legends and  table titles not totally  descriptive.   Graph axes labelled  inappropriately.   References  appropriately with only  minor referencing  issues.   Fonts used are  appropriate    Margins are within  limits   Cover sheet and  declaration have been  completed.   Kept within the page  limit.   Table & figure titles  not in the correct  position.   Figure legend/caption  not present or  inaccurate.   Axis titles not  descriptive or without  units.   Error bars on the  graph are excel  generated (not  representative of  experimental  standard deviation).   Fonts used are  inappropriate    Margins are not  within limits   Have not, or only  partially,  completed the  assignment  cover sheet and  declaration.   Used excel to  label graphs and  placed title in the  wrong position.   Table titles in the  wrong position.   Titles of figures  and tables read  like sentences  rather than  descriptive titles.   Figure axes not  labelled  adequately.   Some  inconsistencies  in referencing  (either within  the text and/or  the reference  list).   Have not  completed the  assignment  cover sheet  and  declaration.   Figures or  tables not  included.   Missing figures,  tables or axis  titles in graphs.   Missing error  bars on graphs.   Exceeded the  page limit.   Have not used  any references.  The laboratory results are  presented in an appropriate  manner. Weight 5%.    https://www.nature.com/scitable/ topicpage/effective-writing- 13815989   Good use of the impersonal  tense (ie didn’t use “I”, “we”  “our” in sentences at all)   Appropriate tenses used  (typically past tense)     Used the word “we”  once or twice   Tenses good   Used the word “I”  once or twice   Tenses good    Riddled with  personal  references   Tenses good   Riddled with  personal  references   Tenses all over  the place.  https://www.nature.com/scitable/topicpage/effective-writing-13815989 https://www.nature.com/scitable/topicpage/effective-writing-13815989 https://www.nature.com/scitable/topicpage/effective-writing-13815989 References are cited in the  prescribed method (i.e. within the  text and in the reference list).  Weight 5%.   Used appropriate  referencing style in text and  for reference list.   Slight inconsistencies  in referencing.   Used referencing  style in text but made  a few errors in the  reference list.    OR vice versa.   Used  referencing but  included author  initials in the ‘in  text’ citation.   Several errors in  referencing  made.   Missing  references in  reference list.   Referenced poor  sources (eg  Wikipedia, text  books)       Did not use  appropriate  referencing  style in text  and/or for  reference list.   Incorrectly  referenced  journal  articles.   No references  The statistical analyses have been  performed correctly. Weight 10%.   Correct t-test  calculations   Correct p-values.    Correct presentation of  p-values in scientific  notation OR, simply  p

Mayank · Accepted Answer

Effect of Curcumin on GST specific enzyme using liver and kidney tissue sample solution
Abstract
The present study aimed to evaluate the role of curcumin as anticancer agent. Its activity was tested against GST specific activity using liver and kidney tissues. 
The solutions of cytosol and mitochondria was taken from liver and kidney tissues as the test solution for curcumin. Standard spectroscopic methods were used for evaluation of the GST specific activity in four groups. Statistical analysis was performed using means with standard deviation (mean ± SD) values. For multiple group comparison, one-way analysis of variance (ANOVA) and Student’s t-test was used followed by post-hoc analysis using Dunnett’s test was used and significant values were set at the level of p≤0.05. 
The results showed that curcumin has significant effect on cytosol as compared with mitochondria in both the tissues. The GST specific activity in terms of U/mg of protein and in liver cytosol group was significantly reduced by 79.24%, while kidney cytosol showed 80.80%, and in kidney mitochondria the activity was slightly reduced by 21.12% in presence of curcumin as compared without curcumin solutions. 
This data suggested that curcumin has significant anti-cancer activity along with antioxidant activity using specific enzyme inhibition mechanisms.  
Introduction
In developing and developed countries, cancer mortality cases have been risen throughout the World and is considered as one of the most leading cause of death in some of the Western countries. This alarming clinical data has continuously increasing the research activities in order to search some complementary and alternative medicine (CAM) approach against cancer and would work as cancer chemo preventive agents, mostly some substance which are mostly included the diet. Turmeric, the dried rhizome was one of the best example to work as chemo protective agent. Curcumin is one of the molecule in the World, which have ‘n’ number of biological activities and research has been continued to identify the mechanism behind the same. Turmeric (diferuloylmethane), a major yellow product obtained from the rhizome of Curcuma longa Linn (Salem, Rohani and Gillies, 2014). Plant is found to have significant protein kinase C inhibition activity, anti-inflammatory, and antioxidant properties. Besides, it has been widely used as a coloring agent in foods, drugs and cosmetics. Approximately, turmeric was used as 4 g/adult/day in various countries, which is one of the reason of low rate of colorectal cancer. It contain variety of curcuminoids and essential oils, which have highest biological activity. It has been reported to inhibit tumor formation in duodenum, skin, forestomach, and mice colon, rat sebaceous glands, tongue, colon, and in mammary glands (Gupta et al., 2012). It worked through various mechanism such as kinases inhibition, activity of transcription factors inhibition like nuclear factor-κB and activator protein-1, and destruction of expression of the enzyme (PERRONE et al., 2015).   
Glutathione S-transferases (GSTs) also called as ligandins, they ate the group of phase II metabolic isozymes include in eukaryotic and prokaryotic. GST are best reported to have their activity as detoxification, by catalyzing the conjugation of the reduced form of glutathione (GSH) into xenobiotic substrates. Cytosolic, mitochondrial, and microsomal are the basic three subfamilies of GST, and they all are extremely diverse in most of the amino acid sequence. It significantly detoxifies most of the endogenous compounds like peroxidised lipids, drugs, pesticides, carcinogens, herbicides, and variably-derived epoxides and enables the breakdown of xenobiotics, which defines to role in cancer. Cytosolic GSTP1-1, one of the GST family member isozyme has significant role in in cancer development and chemotherapeutic resistance.

Metabolic Biochemistry GST and the Effects of Curcumin Practical: Criterion-Referenced Marking Criteria Rubric You will be required to present class data in a laboratory report. In the week following...

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