Module - genetics and molecular biologyTopic - Reverse Transcriptase Polymerase Chain Reaction ( RT-PCR). I want a lab report on this topic with results in table form and proper referencing in it .

Reverse-Transcription Polymerase Chain Reaction
Introduction
Reverse-Transcription Polymerase Chain Reaction (RT-PCR) is a technique in which the complementary DNA or cDNA is synthesised from RNA with the help of an enzyme, Reverse transcriptase and then, the amplification of specific DNA targets using Polymerase Chain Reaction (PCR). During 1970s discovery of retroviral reverse transcriptase David Baltimore and Howard Temin made RT-PCR possible (Coffin and Fan, 2016). Reverse transcriptase is a RNA-dependent DNA polymerase enzyme which is primarily found in retroviruses such as Human Immunodeficiency virus (HIV), Avian Myeloblastosis Virus (AMV) etc. The reverse transcriptase synthesises DNA by using RNA as a template and hence it is considered as RNA-dependent DNA polymerase. This enzyme helps to synthesise DNA from the RNA of virus which then incorporates with the host genome. The main purpose of RT-PCR is to quantify the RNA level as the DNA has both coding and non-coding sequences which has exons, introns etc. The exons encode the final mRNA, which is further used in the process of translation of amino acids. Hence, by quantifying mRNA, one can quantify the number of genes present in a sample. RT-PCR was used to quantify the RNA levels in the given sample with the help of a fluorescent dye (Livak and Schmittgen, 2001). The RNA is unstable and hence, making cDNA from RNA prevents it from RNase degradation. For cDNA synthesis, Primers, Reverse transcriptase enzyme and RNA as a template is required in vitro. The cDNA synthesis can be done using oligo dT primers or Random hexamer primers or it can be synthesised using strand-specific or gene-specific primers. The further amplification of cDNA was carried out by gene-specific primers and using Taq polymerase enzyme. An appropriate designing of primers provides thermodynamic stability, specificity to the template supports the RT-PCR (Jalali, Zaborowska and Jalali, 2017). RT-PCR has wide applications in the field of medicine. It is used to detect various viral diseases such as COVID-19. It is also used to check the level of gene expression in normal and cancerous cells (Bachman, 2013). It is used to calculate the viral load and expression levels. It determines the tissue-specific gene expression and measure the expression of tissue-specific mutant alleles. Gene therapy and gene insertion studies also used RT-PCR techniques. It is also required in single nucleotide polymorphism (SNPs) analysis, chromosome aberrations analysis...