Thank you so much for your support, this is a Systems Biology RNA sequencing report that is due tomorrow 11.59pm. It requires the access to Galaxy Australia which I will provide shortly.

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Thank you so much for your support, this is a Systems Biology RNA sequencing report that is due tomorrow 11.59pm. It requires the access to Galaxy Australia which I will provide shortly.


Microsoft Word - BIOL2499:BIOL2512 2021 Practical Report 2 Assessment details.docx BIOL2499/BIOL2512 2022 Assessment: Practical Report 2 (RNA-Seq) Assessment details – Course: Systems Biology Assessment task and purpose To provide an opportunity for students to demonstrate technical ability in the critical analysis and interpretation of omics data. Course learning outcomes (CLO) This assessment relates to the following CLOs 1. Demonstrate theoretical and technical understanding of Systems Biology (Omics) approaches to the study of biological systems from the level of molecules to cells, tissues, organisms and populations. 2. Critically analyse, interpret and explain omics data. 3. Summarise and synthesize and evaluate primary scientific literature. 4. Communicate clearly and effectively using correct scientific language and conventions. Assessment description / requirements Working as an individual, use the instructions in the practical manual to complete the practical activity for week 6. As an individual, complete the questions relating to the assessment criteria. This assessment will demonstrate a capacity to evaluate complex ideas and concepts in research of omics datasets. Assessment type & weighting This is a summative assessment. This assessment is worth 10% of the total mark for this course. Assessment submission details Deadline: Midnight Sunday the 15th of May Method: Submit the assessment onto CANVAS by the due date. The file format should be Word or pdf. Maximum 1000 words. Grading / performance standards A rubric is available on CANVAS. It outlines the grading and performance standards. Students are encouraged to read the rubric in conjunction with the assessment description. The Discussion Board provides opportunities for Q&A about grading and performance throughout the assessment period. Assessment preparation/ Resources https://usegalaxy.org.au/ Assessment feedback A rubric will be used to grade individual performance. Assessment feedback will be provided to individuals on CANVAS at the conclusion of the marking period. Students can ask for input (feedback) throughout the assessment period using the Discussion Board’s Q&A. In addition, at the conclusion of the marking period, video feedback addressing the strengths and weaknesses of all students’ performance (collective feedback) will be provided. Indicators of authentic and blended assessment Authentic in this assessment • Relates to world-wide trends e.g. commonly used techniques in systems biology • Requires students to use technology in a practical context Blended in this assessment • Digital technology Microsoft Word - BIOL2499:BIOL2512 2021 Practical Report 2 Assessment details.docx BIOL2499/BIOL2512 2022 Assessment: Practical Report 2 (RNA-Seq) Assessment details – Course: Systems Biology Assessment task and purpose To provide an opportunity for students to demonstrate technical ability in the critical analysis and interpretation of omics data. Course learning outcomes (CLO) This assessment relates to the following CLOs 1. Demonstrate theoretical and technical understanding of Systems Biology (Omics) approaches to the study of biological systems from the level of molecules to cells, tissues, organisms and populations. 2. Critically analyse, interpret and explain omics data. 3. Summarise and synthesize and evaluate primary scientific literature. 4. Communicate clearly and effectively using correct scientific language and conventions. Assessment description / requirements Working as an individual, use the instructions in the practical manual to complete the practical activity for week 6. As an individual, complete the questions relating to the assessment criteria. This assessment will demonstrate a capacity to evaluate complex ideas and concepts in research of omics datasets. Assessment type & weighting This is a summative assessment. This assessment is worth 10% of the total mark for this course. Assessment submission details Deadline: Midnight Sunday the 15th of May Method: Submit the assessment onto CANVAS by the due date. The file format should be Word or pdf. Maximum 1000 words. Grading / performance standards A rubric is available on CANVAS. It outlines the grading and performance standards. Students are encouraged to read the rubric in conjunction with the assessment description. The Discussion Board provides opportunities for Q&A about grading and performance throughout the assessment period. Assessment preparation/ Resources https://usegalaxy.org.au/ Assessment feedback A rubric will be used to grade individual performance. Assessment feedback will be provided to individuals on CANVAS at the conclusion of the marking period. Students can ask for input (feedback) throughout the assessment period using the Discussion Board’s Q&A. In addition, at the conclusion of the marking period, video feedback addressing the strengths and weaknesses of all students’ performance (collective feedback) will be provided. Indicators of authentic and blended assessment Authentic in this assessment • Relates to world-wide trends e.g. commonly used techniques in systems biology • Requires students to use technology in a practical context Blended in this assessment • Digital technology Microsoft Word - BIOL2499:BIOL2512 2022 Practical Report 2 Assessment Guidelines.docx BIOL2499/BIOL2512 Systems Biology – Practical Report 2 1 BIOL2499/BIOL2512 Practical Report 2 Use your results from Practical Activity 2: RNA-Seq: Differential Gene Expression (week 6) to complete this assessment using a standard practical report structure: Introduction (brief), Aim, Results, Methods (just refer to the practical manual in this section) and Discussion. The focus should be on the results and discussion sections. Maximum 1000 words (not including references and figure legends) Include answers to the following questions in the results section of your report: The Importance of Replicates: How many differentially expressed genes with adjusted p-value < 0.05="" are="" there?="" how="" many="" differentially="" expressed="" genes="" can="" you="" identify="" using="" 2="" samples="" in="" each="" group="" and="" an="" adjusted="" p-value="" of=""><0.05? how="" many="" differentially="" expressed="" genes="" can="" you="" identify="" using="" only="" 1="" sample="" in="" each="" group="" and="" an="" adjusted="" p-value="" of=""><0.05? include answers to the following questions in the discussion section of your report: alignment of reads from rna-seq: what is the major challenge faced when aligning reads from an rna-seq experiment to a reference genome sequence? how is this challenge overcome? the importance of replicates: use your results to explain why it is important to have biological replicates in an rna-seq experiment. include="" answers="" to="" the="" following="" questions="" in="" the="" discussion="" section="" of="" your="" report:="" alignment="" of="" reads="" from="" rna-seq:="" what="" is="" the="" major="" challenge="" faced="" when="" aligning="" reads="" from="" an="" rna-seq="" experiment="" to="" a="" reference="" genome="" sequence?="" how="" is="" this="" challenge="" overcome?="" the="" importance="" of="" replicates:="" use="" your="" results="" to="" explain="" why="" it="" is="" important="" to="" have="" biological="" replicates="" in="" an="" rna-seq="">
Answered 2 days AfterMay 28, 2022

Answer To: Thank you so much for your support, this is a Systems Biology RNA sequencing report that is due...

Dr Shweta answered on May 30 2022
78 Votes
RNA sequencing- Differential gene expression
Introduction
Ribonucleic acid (RNA) is a single-stranded nucleic acid which is present in all living cells. RNA molecule is made up of phosphate groups, ribose sugar and nucleotides but exce
pt Thymine RNA contains Uracil. RNA is formed from the DNA by the process of transcription. The main function of RNA in cell is to synthesize proteins by the process of translation. RNA carries the genetic information which is processed into the ribosomes into different types of proteins necessary for the various cellular processes. There are three main types of RNA – the mRNA, rRNA, and the tRNA which are involved in the process of protein synthesis. Differential expression of different segments of RNA is basically the differential gene expression that regulates the cell differentiation. RNA sequencing is a sequencing method used for the identification and quantification of RNA in the given sample with the help of Next-generation sequencing technology. Next-generation sequencing (NGS) technologies are transforming the genome research and can be used to tackle the de novo sequencing of large genomes. NSG more specifically used in transcriptomics analysis and it use is progressively increasing for studying the gene expression profile since it is capable to account for the individual genome differences within the same species. The characteristics of RNA-seq data analysis by sequencing depth helps in the detection of differentially expressed transcripts using the transcript biotype, length of sequence, expression level of genes and fold-changes. The true potential of RNA-seq is its ability to study the gene regulations even at the low expression ranges. RNA-seq is widely used for the study of differential gene expression patterns. Differential gene expression means the expressions of different segments of same DNA in different cells or different expression of transcriptome which basically is the root cause of difference in different cells and formation of different organs.
Objective - In this practical activity we use the transcriptome analysis data of common fruit fly, Drosophila melanogaster and by its analysis through HISAT tool of Galaxy system, we will understand the process of how the differentially expressed genes can be identified using RNA-Sequencing (RNA-Sequencing Differential Gene...
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